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beta Actin antibody - VetSignal

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932
Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134932
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932
Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134932
Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134932
Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134932

Cat No. GTX134932

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Cat, Dog
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog beta Actin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

actin beta

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Database

Research Area

DATA IMAGES
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932

GTX134932 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody (GTX134932) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134932

GTX134932 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX134932) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932

GTX134932 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
beta Actin stained by beta Actin antibody (GTX134932) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134932

GTX134932 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
beta Actin stained by beta Actin antibody (GTX134932) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134932

GTX134932 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX134932) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134932

GTX134932 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX134932) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134932

GTX134932 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX134932) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319