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beta Actin antibody

KO/KD
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313

Cat. No. GTX100313

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Drosophila, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3 , Raji , 293T

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
Beta-actin antibody
GTX100313 diluted at 1:1000

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX100313 diluted at 1:5000

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
beta Actin antibody (GTX100313) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100313

GTX100313 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin(GTX100313) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Act5C is the homolog of mammalian beta Actin. (Gene ID: 31521/ UniProt: P10987)

REFERENCE

Application Reference

Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.
Lin JW et al. Cell Death Discov 2021; 7 (1) : 35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Christen KE et al. Int J Biochem Cell Biol 2019; 112 : 24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.
Application : WB
Reactivity : Human
Li PH et al. Eur J Med Chem 2018; 145 : 498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.
Application : WB
Reactivity : Human
Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
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REVIEW

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Application Reference
Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.
Lin JW et al. Cell Death Discov 2021; 7 (1):35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Christen KE et al. Int J Biochem Cell Biol 2019; 112:24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.
Application : WB
Reactivity : Human
Li PH et al. Eur J Med Chem 2018; 145:498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.
Application : WB
Reactivity : Human
Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Application : WB
Reactivity : Human
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169