GeneTex
United States (US)

beta Actin antibody

KO/KD
GTX100313_40177_IHC_19011506_694.jpg
GTX100313_40177_IHC_M_19011506_620.jpg
GTX100313_42753_20170419_IFA_19011506_732.jpg
GTX100313_WB_19011506_706.jpg
GTX100313_42305_20160707_WB_shRNA_watermark_19011506_733.jpg
GTX100313_42753_20170209_WB_19011506_273.jpg
GTX100313_40177_WB_M_19011506_261.jpg

Cat No. GTX100313

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji , NIH-3T3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.53mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PS1TP5BP1 antibody, ACTB antibody, "actin, cytoplasmic 1 antibody", beta cytoskeletal actin antibody, PS1TP5-binding protein 1 antibody, "actin, beta antibody"

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100313_40177_IHC_19011506_694.jpg

GTX100313 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin(GTX100313) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100313_40177_IHC_M_19011506_620.jpg

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
beta Actin antibody (GTX100313) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100313_42753_20170419_IFA_19011506_732.jpg

GTX100313 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX100313) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100313_WB_19011506_706.jpg

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
Beta-actin antibody
GTX100313 diluted at 1:1000

GTX100313_42305_20160707_WB_shRNA_watermark_19011506_733.jpg

GTX100313 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.

GTX100313_42753_20170209_WB_19011506_273.jpg

GTX100313 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000.

GTX100313_40177_WB_M_19011506_261.jpg

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX100313 diluted at 1:5000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169