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beta Actin antibody

KO/KD
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100313
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313
Anti-beta Actin antibody used in Western Blot (WB). GTX100313

Cat. No. GTX100313

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Drosophila, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3 , Raji , 293T

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174236

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
beta Actin antibody (GTX100313) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
Beta-actin antibody
GTX100313 diluted at 1:1000

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX100313 diluted at 1:5000

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100313

GTX100313 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

GTX100313 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin(GTX100313) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Act5C is the homolog of mammalian beta Actin. (Gene ID: 31521/ UniProt: P10987)

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

GTX100313 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.
Lin JW et al. Cell Death Discov 2021; 7 (1) : 35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Christen KE et al. Int J Biochem Cell Biol 2019; 112 : 24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.
Application : WB
Reactivity : Human
Li PH et al. Eur J Med Chem 2018; 145 : 498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.
Application : WB
Reactivity : Human
Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
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REVIEW

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Application Reference
Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.
Lin JW et al. Cell Death Discov 2021; 7 (1):35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Christen KE et al. Int J Biochem Cell Biol 2019; 112:24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.
Application : WB
Reactivity : Human
Li PH et al. Eur J Med Chem 2018; 145:498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.
Application : WB
Reactivity : Human
Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.
Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.
Application : WB
Reactivity : Human
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169