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beta Actin antibody

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100315
Anti-beta Actin antibody used in Western Blot (WB). GTX100315
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100315
Anti-beta Actin antibody used in Western Blot (WB). GTX100315

Cat. No. GTX100315

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100315

GTX100315 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX100315) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Actin antibody used in Western Blot (WB). GTX100315

GTX100315 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100315) diluted at 1:1000.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100315

GTX100315 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
beta Actin antibody (GTX100315) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX100315

GTX100315 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100315) diluted at 1:1000.

REFERENCE

Application Reference

Li CJ et al. Evid Based Complement Alternat Med 2012; 2012 : 261971 Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169