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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639

Cat No. GTX109639

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377395)

Predict Reactivity

Rabbit, Bovine, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: NTH-3T3
C: mouse brain
D: PC-12
E: Rat brain
F: drosophila
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (whole cell lysate)
A: Candida albicans (sc5314) 20ug
B: Candida albicans (sc5314) 40ug
10% SDS PAGE
GTX109639 diluted at 1:4000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639

GTX109639 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin(GTX109639) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: Jurkat
D: Raji
10% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
B. 50 μg mouse kidney lysate/extract
C. 50 μg mouse lung lysate/extract
D. 50 μg mouse testis lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
beta Actin antibody (GTX109639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
beta Actin antibody (GTX109639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Snail1 loss altered cytoskeletal organization and gene expressions in RMG1 cells.(A) Representative images displaying the cell morphology of wild-type (WT) RMG cells (I), Snail1 KO1 cells (II). Cells are visualized by phase-contrast microscopy. Scale bar indicates 50 μm. WT cells exhibited cobblestone-like morphologies, whereas most of the Snail1 KO1 cells exhibit a more rounded morphology. Representative images of the actin cytoskeletons of WT cells (III) Snail1 KO1 cells (IV) stained with rhodamine X-conjugated phalloidine and visualized using a confocal laser scanning microscope (Zeiss LSM700). Photos of 5 to 10 images per sample were taken. Experiments were repeated 5 times. WT cells are rich in stress fibers, whereas Snail1 KO1 cells have scarce stress fibers, but cortical actin on their cell surfaces. Scale bar indicates 20 μm. (B) Quantitative RT-PCR of indicated genes in wild-type (WT) RMG cells, Snail1 KO1 cells, and Snail1 KO1 cells transiently transfected with a Snail1 expression vector (Snail1 KO1 + Snail1 cells). Values are expressed as the mean ± s.e.m of triplicate samples. Experiments were repeated three times. Statistical significance is indicated by an asterisk (*P < 0.05 and ** P < 0.01 using Students’t-test). Snail1 KO1 cells showed a complete loss of Snail expression and increased expression of E-cadherin, claudin-1, occludin, beta-actin, and alpha-tubulin, as well as decreased expression of integrin alpha 5. Transient expression of Snail1 in Snail1 KO cells reversed these effects, except for the increase in occludin expression. (C) Western blot analysis of indicated proteins in wild-type (WT) RMG cells, Snail1 KO1 cells, and Snail1 KO1 cells transiently transfected with a Snail1 expression vector (Snail1 KO1 + Snail1 cells). Snail1 KO1 cells showed a complete loss of Snail1 expression and an increase in E-cadherin, occludin and claudin-1, beta-actin, alpha- tubulin, as well as a decreased in integrin alpha 5 expression. Transient expression of Snail1 in Snail1 KO cells reversed these effects, except for the decreased in integrin alpha 5 expression. Vinculin was used as a loading control. Experiments were repeated three times.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Mutant HTT aggregation in the brain of R6/2 mice deleted for one Supt4h allele.(A) Wild-type HTT protein levels were examined by Western blot analysis using brain lysates collected from right cerebrum of animals as described in Materials and Methods and in Fig 3B. TATA-binding protein (TBP) produced by a gene containing 13–15 consecutive CAA/CAG repeats was also analyzed. β-actin served as loading control. (B) Brain lysates collected from 12-week-old mice were loaded onto a cellulose acetate CA membrane, which traps only aggregated protein. Mutant HTT protein was detected using EM48 antibody. Nitrocellulose (NC) membranes were employed for slot blot assays to determine protein abundance; α-Tubulin served as a loading control. The values shown are means ± SEM, and the relative protein aggregation in tissues of R6/2 HD mice having two or one allele of Supt4h is presented in the bottom panel. (C) Representative IHC images of cerebral tissue of 12-week-old R6/2 (HD) mice having either one or two alleles of Supt4h are shown. HTT aggregates were detected using an antibody against ubiquitin, which is recruited to and co-localized with aggregates in the brain of HD mice [54]. The positions of aggregates are indicated by arrowheads. (D) DARPP-32 protein abundance was analyzed by Western blot analysis using brain lysates collected from R6/2 mice at the age of 12 weeks either intact in the Supt4h locus or deleted for one Supt4h allele. The level of WT mice having two Supt4h alleles was set to 1, after normalization with α-Tubulin. Data are presented as the mean ± SEM (n = 3 in each group; *, p < 0.05 by Student’s t-test).

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
10% SDS PAGE
GTX109639 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639

GTX109639 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Beta-actin (GTX109639) antibody at 1:500 dilution.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Beta actin(GTX109639) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (10 μg of whole cell lysate)
A: Yeast lysate
7.5% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

beta Actin antibody Cat No. GTX109639

Rating ( Average 4.7 based on 11 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 11 users reviews)
Date : Beished Shabangu submitted on 17-Nov-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Blocking : 5% Milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 1Hr
Date : Anonymous submitted on 29-Oct-2019
Score :
Application Tested : WB
Sample Species : Sheep
Sample : Endometrium extracts
Amount used : 30 μg
Blocking : Block Ace (KAC Co., Ltd.), 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 17-Dec-2015
Score :
Application Tested : WB
Sample Species : Cat
Sample : Mya-1 cells
Amount used : 60μg
Blocking : 5% milk, 22°C, 1Hr
Primary Antibody : 1:5000, 22°C, 1Hr
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human Periodontal ligament cells
Amount used : 10μg
Blocking : ECL Prime Blocking Reagent, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : ileum tissue, heart tissue, skeletal muscle tissue, brain tissue
Amount used : 50-100 μg
Blocking : 5% skim milk in PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 37°C, 0.5Hr
Lane Description : Lane 1: 100ug mouse ileum tissue, Lane 2: 50ug mouse heart tissue, Lane 3: 100ug mouse skeletal muscle tissue, Lane 4: 50ug mouse brain tissue
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Date : Anonymous submitted on 07-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : H9C2 cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse aorta tissue
Amount used : 4μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 22-Dec-2011
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Rice
Sample : Rice leaf
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf