GeneTex
United States (US)

beta Actin antibody

GTX109639_40317_WB_multi species_19011720_350.jpg
GTX109639_40317_CT_WB_19011507_878.jpg
GTX109639_39967_IFA_19011507_652.jpg
GTX109639_40317_WB_19011507_177.jpg
GTX109639_40317_WB_M_19011507_526.jpg
GTX109639_40317_WB_multi cell line_19011720_605.jpg
GTX109639_42438_20160504_IHC-P_R_19011507_477.jpg
GTX109639_41899_20160707_WB_shRNA_watermark_19011507_716.jpg
GTX109639_42466_20160511_IHC-P_M_19011507_978.jpg
GTX109639_40317_WB_sensitive_19011507_227.jpg
GTX109639_IFA_19011507_221.jpg
GTX109639_IHC_19011507_212.jpg
GTX109639_40436_WB_R_19011507_958.jpg
GTX109639_40317_WB_Y_19011507_904.jpg

Cat No. GTX109639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Yeast, Drosophila, Cat, Dog, Hamster, Pig, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377395)

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX109639_40317_WB_multi species_19011720_350.jpg

GTX109639 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: NTH-3T3
C: mouse brain
D: PC-12
E: Rat brain
F: drosophila
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_40317_CT_WB_19011507_878.jpg

GTX109639 WB Image

Sample (whole cell lysate)
A: Candida albicans (sc5314) 20ug
B: Candida albicans (sc5314) 40ug
10% SDS PAGE
GTX109639 diluted at 1:4000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_39967_IFA_19011507_652.jpg

GTX109639 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin(GTX109639) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX109639_40317_WB_19011507_177.jpg

GTX109639 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: Jurkat
D: Raji
10% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_40317_WB_M_19011507_526.jpg

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
B. 50 µg mouse kidney lysate/extract
C. 50 µg mouse lung lysate/extract
D. 50 µg mouse testis lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_40317_WB_multi cell line_19011720_605.jpg

GTX109639 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_42438_20160504_IHC-P_R_19011507_477.jpg

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
beta Actin antibody (GTX109639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109639_41899_20160707_WB_shRNA_watermark_19011507_716.jpg

GTX109639 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_42466_20160511_IHC-P_M_19011507_978.jpg

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
beta Actin antibody (GTX109639) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109639_40317_WB_sensitive_19011507_227.jpg

GTX109639 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
10% SDS PAGE
GTX109639 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_IFA_19011507_221.jpg

GTX109639 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Beta-actin (GTX109639) antibody at 1:500 dilution.

GTX109639_IHC_19011507_212.jpg

GTX109639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Beta actin(GTX109639) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109639_40436_WB_R_19011507_958.jpg

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109639_40317_WB_Y_19011507_904.jpg

GTX109639 WB Image

Sample (10 µg of whole cell lysate)
A: Yeast lysate
7.5% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

beta Actin antibody Cat No. GTX109639

Rating ( Average 4.7 based on 9 users reviews)
Application
Western Blot(WB) ( Average 4.7 based on 9 users reviews)
Date : Anonymous submitted on 17-Dec-2015
Score :
Application Tested : WB
Sample Species : Cat
Sample : Mya-1 cells
Amount used : 60μg
Blocking : 5% milk, 22°C, 1Hr
Primary Antibody : 1:5000, 22°C, 1Hr
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human Periodontal ligament cells
Amount used : 10μg
Blocking : ECL Prime Blocking Reagent, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : ileum tissue, heart tissue, skeletal muscle tissue, brain tissue
Amount used : 50-100 μg
Blocking : 5% skim milk in PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 37°C, 0.5Hr
Lane Description : Lane 1: 100ug mouse ileum tissue, Lane 2: 50ug mouse heart tissue, Lane 3: 100ug mouse skeletal muscle tissue, Lane 4: 50ug mouse brain tissue
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Date : Anonymous submitted on 07-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : H9C2 cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse aorta tissue
Amount used : 4μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 22-Dec-2011
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Rice
Sample : Rice leaf
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169