beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.
*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament
IP/MS validation was supported by references (PMID:30377395)
Rabbit, Bovine, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.13 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
actin beta , BRWS1 , PS1TP5BP1
Cytoplasm , cytoskeleton
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]