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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in Western Blot (WB). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

Cat No. GTX109639

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

beta-Actin antibody detects beta-actin, a cytoskeletal protein with a predicted molecular weight of 42 kDa. Because of its widespread expression, beta-actin is commonly used as a loading control for various protein assays, particularly western blots, to estimate sample protein amount and quality.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament

Product Note

IP/MS validation was supported by references (PMID:30377395)

Predict Reactivity

Rabbit, Bovine, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949572

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: NTH-3T3
C: mouse brain
D: PC-12
E: Rat brain
F: drosophila
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (whole cell lysate)
A: Candida albicans (sc5314) 20ug
B: Candida albicans (sc5314) 40ug
10% SDS PAGE
GTX109639 diluted at 1:4000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: Jurkat
D: Raji
10% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
B. 50 μg mouse kidney lysate/extract
C. 50 μg mouse lung lysate/extract
D. 50 μg mouse testis lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
10% SDS PAGE
GTX109639 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
10% SDS PAGE
GTX109639 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Beta actin(GTX109639) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX109639) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Sample (10 μg of whole cell lysate)
A: Yeast lysate
7.5% SDS PAGE
GTX109639 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX109639

GTX109639 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109639

GTX109639 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX109639

GTX109639 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
beta Actin stained by beta Actin antibody (GTX109639) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

beta Actin antibody Cat No. GTX109639

Rating ( Average 5 based on 83 users reviews)
Application
Western Blot(WB) ( Average 5 based on 82 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : IHC-P
Sample Species : Chk
Sample : Chicken Ileum
Blocking : 10% normal goat serum, 20min, 25°C
Fixation : Bouin’s fixative
Primary Antibody : 1:5000, 25°C, 24Hr
Figure Note : Microvilli showing beta-actin immunoreactivity (arrows) are found in the apical surface of enterocytes in the chicken ileum.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31409793
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2016.PMID: 27831565
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31744099
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2016.PMID: 27918551
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal BMC Nephrol in 2018.PMID: 30005632
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28169314
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Clin Med in 2019.PMID: 31167519
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31501500
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Exp Ther Med in 2014.PMID: 24396418
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2020.PMID: 32286426
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31744099
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Int Soc Sports Nutr in 2015.PMID: 25792976
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2018.PMID: 29361943
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Aging Dis in 2019.PMID: 31164994
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2016.PMID: 27918551
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Aging Dis in 2019.PMID: 31164994
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23691025
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31744099
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31744099
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2016.PMID: 27918551
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2016.PMID: 27918551
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23691025
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2018.PMID: 29337987
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2018.PMID: 29337987
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31744099
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2016.PMID: 27918551
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31409793
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal ScientificWorldJournal in 2014.PMID: 24578622
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2017.PMID: 28926934
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27281159
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27404495
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2015.PMID: 25760041
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28325912
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 29016672
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2017.PMID: 28472954
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2017.PMID: 28472954
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2017.PMID: 28472954
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25815818
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25815818
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2017.PMID: 28472954
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25815818
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25815818
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26161782
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 26838546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013.PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013.PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013.PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013.PMID: 23788038
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2017.PMID: 28837077
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Pig
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24533157
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2018.PMID: 29789400
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2015.PMID: 25869099
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019.PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29968775
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29968775
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncogenesis in 2015.PMID: 26690546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Rat
Figure Note : The data was peer reviewed and published in the journal J Cancer in 2019.PMID: 31762816
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2015.PMID: 25869099
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31323961
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31323961
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31323961
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31323961
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Materials (Basel) in 2018.PMID: 30223515
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25437003
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27036017
Date : Beished Shabangu submitted on 17-Nov-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Blocking : 5% Milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 1Hr
Date : Anonymous submitted on 29-Oct-2019
Score :
Application Tested : WB
Sample Species : Sheep
Sample : Endometrium extracts
Amount used : 30 μg
Blocking : Block Ace (KAC Co., Ltd.), 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 17-Dec-2015
Score :
Application Tested : WB
Sample Species : Cat
Sample : Mya-1 cells
Amount used : 60μg
Blocking : 5% milk, 22°C, 1Hr
Primary Antibody : 1:5000, 22°C, 1Hr
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human Periodontal ligament cells
Amount used : 10μg
Blocking : ECL Prime Blocking Reagent, 25°C, 0.5Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : ileum tissue, heart tissue, skeletal muscle tissue, brain tissue
Amount used : 50-100 μg
Blocking : 5% skim milk in PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 37°C, 0.5Hr
Lane Description : Lane 1: 100ug mouse ileum tissue, Lane 2: 50ug mouse heart tissue, Lane 3: 100ug mouse skeletal muscle tissue, Lane 4: 50ug mouse brain tissue
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Date : Anonymous submitted on 07-Jan-2015
Score :
Application Tested : WB
Sample Species : Rat
Sample : H9C2 cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse aorta tissue
Amount used : 4μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 22-Dec-2011
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Rice
Sample : Rice leaf
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf