Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver , rat liver , PC-12 , Rat2 , drosophila
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618080
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
actin beta , BRWS1 , PS1TP5BP1
Cellular Localization
Cytoplasm , cytoskeleton
Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Database
Research Area