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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Immunoprecipitation (IP). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564

Cat No. GTX110564

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat, Drosophila
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver , rat liver , PC-12 , Rat2 , drosophila

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 µg drosophila lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Immunoprecipitation (IP). GTX110564

GTX110564 IP Image

Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564).
Western blot analysis was performed using beta Actin antibody (GTX110564).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110564

GTX110564 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Various whole cell extracts (15 | 5 | 2 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169