APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
rat liver , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.46 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618080
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actin beta , BRWS1 , PS1TP5BP1
Cellular Localization
Cytoplasm , cytoskeleton
Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX110564 WB Image
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX110564 WB Image
beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX110564 WB Image
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 IP Image
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564).
Western blot analysis was performed using beta Actin antibody (GTX110564).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
|
GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX110564 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX110564 WB Image
Yeast lysate (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 ICC/IF Image
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX110564 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX10564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX110564 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
REVIEW
beta Actin antibody
Cat. No. GTX110564
|
Rating
|
|
( Average 5 based on 8 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 5 based on 8 users reviews)
|
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Front Physiol in 2017.
PMID: 28588500
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2016.
PMID: 27486856
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Redox Biol in 2014.
PMID: 24936442
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging Cell in 2017.
PMID: 27623715
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging Cell in 2017.
PMID: 27623715
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging Cell in 2017.
PMID: 27623715
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2019.
PMID: 30818369
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS Pathog in 2019.
PMID: 30818369
