GeneTex
United States (US)

beta Actin antibody

GTX124212_40758_20141024_IHC_M_18121410_894.jpg
GTX124212_40758_WB_M_18121410_259.jpg
GTX124212_41164_20170205_IHC-P_M_18121410_834.jpg
GTX124212_41164_WB_Sensitive_18121410_719.jpg
GTX124212_41808_20170205_IHC-P_M_2_18121410_414.jpg
GTX124212_40758_20170205_IHC-P_M_2_18121410_485.jpg
GTX124212_41808_20170205_IHC-P_M_1_18121410_708.jpg
GTX124212_41164_20170205_IHC-P_R_2_18121410_864.jpg
GTX124212_41808_20170205_IHC-P_R_18121410_200.jpg
GTX124212_41164_20170205_IHC-P_R_1_18121410_731.jpg
GTX124212_40758_20170205_IHC-P_M_1_18121410_846.jpg
GTX124212_40758_20170205_IHC-P_R_2_18121410_778.jpg
GTX124212_41808_20161123_IFA_18121410_517.jpg
GTX124212_41808_20141107_WB_18121410_676.jpg
GTX124212_40758_20170205_IHC-P_R_1_18121410_515.jpg

Cat No. GTX124212

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124212_40758_20141024_IHC_M_18121410_894.jpg

GTX124212 IHC-P Image

beta Actin antibody detects beta Actin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
beta Actin antibody (GTX124212) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124212_40758_WB_M_18121410_259.jpg

GTX124212 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX124212 diluted at 1:10000

GTX124212_41164_20170205_IHC-P_M_18121410_834.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41164_WB_Sensitive_18121410_719.jpg

GTX124212 WB Image

Beta Actin antibody detects beta Actin protein by Western blot analysis.
A. 1 μg 293T whole cell lysate/extract
10 % SDS-PAGE
beta Actin antibody (GTX124212) dilution: 1:10000

GTX124212_41808_20170205_IHC-P_M_2_18121410_414.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_40758_20170205_IHC-P_M_2_18121410_485.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41808_20170205_IHC-P_M_1_18121410_708.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41164_20170205_IHC-P_R_2_18121410_864.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41808_20170205_IHC-P_R_18121410_200.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41164_20170205_IHC-P_R_1_18121410_731.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_40758_20170205_IHC-P_M_1_18121410_846.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_40758_20170205_IHC-P_R_2_18121410_778.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124212_41808_20161123_IFA_18121410_517.jpg

GTX124212 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX124212) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX124212_41808_20141107_WB_18121410_676.jpg

GTX124212 WB Image

beta Actin antibody detects beta Actin protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124212) diluted by 1:10000.

GTX124212_40758_20170205_IHC-P_R_1_18121410_515.jpg

GTX124212 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Beta Actin antibody (GTX124212) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169