GeneTex
United States (US)

beta Actin antibody

KO/KD
GTX124213_40751_WB_18121410_311.jpg
GTX124213_40751_WB_R_18121410_939.jpg
GTX124213_40751_IHC_18121410_944.jpg
GTX124213_41808_20161123_IFA_18121410_615.jpg
GTX124213_40751_WB_M_18121410_871.jpg
GTX124213_40751_20170205_IHC-P_R_18121410_737.jpg
GTX124213_40751_20160707_WB_shRNA_watermark_18121410_959.jpg
GTX124213_41808_20170205_IHC-P_R_18121410_970.jpg
GTX124213_40751_IHC_R_18121410_656.jpg
GTX124213_40751_20160401_IP_18121410_482.jpg

Cat No. GTX124213

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124213_40751_WB_18121410_311.jpg

GTX124213 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX124213 diluted at 1:10000

GTX124213_40751_WB_R_18121410_939.jpg

GTX124213 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX124213 diluted at 1:10000

GTX124213_40751_IHC_18121410_944.jpg

GTX124213 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta Actin(GTX124213) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124213_41808_20161123_IFA_18121410_615.jpg

GTX124213 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX124213) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX124213_40751_WB_M_18121410_871.jpg

GTX124213 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX124213 diluted at 1:10000

GTX124213_40751_20170205_IHC-P_R_18121410_737.jpg

GTX124213 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
Beta Actin antibody (GTX124213) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124213_40751_20160707_WB_shRNA_watermark_18121410_959.jpg

GTX124213 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124213) diluted at 1:15000.

GTX124213_41808_20170205_IHC-P_R_18121410_970.jpg

GTX124213 IHC-P Image

Beta Actin antibody detects beta Actin protein at cytoplasm in rat thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
Beta Actin antibody (GTX124213) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124213_40751_IHC_R_18121410_656.jpg

GTX124213 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using beta Actin(GTX124213) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124213_40751_20160401_IP_18121410_482.jpg

GTX124213 IP Image

Immunoprecipitation of beta Actin protein from A431 whole cell extracts using 5 μg of beta Actin antibody (GTX124213).
Western blot analysis was performed using beta Actin antibody (GTX124213).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169