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beta Actin antibody

KO/KD
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124214

Cat. No. GTX124214

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3 , Jurkat , Raji , K562 , 293T

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177721

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Act5C is the homolog of mammalian beta Actin. (Gene ID: 31521/ UniProt: P10987)

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000.

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: Jurakt
B: Raji
C: K562
10% SDS PAGE
GTX124214 diluted at 1:10000

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX124214 diluted at 1:10000

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214

GTX124214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta actin(GTX124214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214

GTX124214 IHC-P Image

beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta actin antibody (GTX124214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124214

GTX124214 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using beta actin(GTX124214) antibody at 1:500 dilution.

REFERENCE

Application Reference

De Tomi E et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci 2022; 23 (3) Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Jacobs CL et al. Nat Methods 2018; 15 (7) : 523-526 StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins.
Peeva V et al. Nat Commun 2018; 9 (1) : 1727 Linear mitochondrial DNA is rapidly degraded by components of the replication machinery.
Application : WB
Reactivity : Human
Chung HK et al. Nat Chem Biol 2015; 11 (9) : 713-20 Tunable and reversible drug control of protein production via a self-excising degron.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169