GeneTex
United States (US)

beta Actin antibody

KO/KD
GTX124214_40751_20160707_WB_shRNA_watermark_18121410_814.jpg
GTX124214_40751_WB_M_18121410_248.jpg
GTX124214_40751_IHC_M_18121410_198.jpg
GTX124214_40751_IFA_18121410_687.jpg
GTX124214_40751_WB_18121410_826.jpg
GTX124214_40751_IHC_18121410_480.jpg

Cat No. GTX124214

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , Jurkat , Raji , K562 , NIH-3T3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124214_40751_20160707_WB_shRNA_watermark_18121410_814.jpg

GTX124214 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000.

GTX124214_40751_WB_M_18121410_248.jpg

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX124214 diluted at 1:10000

GTX124214_40751_IHC_M_18121410_198.jpg

GTX124214 IHC-P Image

beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta actin antibody (GTX124214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124214_40751_IFA_18121410_687.jpg

GTX124214 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using beta actin(GTX124214) antibody at 1:500 dilution.

GTX124214_40751_WB_18121410_826.jpg

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: Jurakt
B: Raji
C: K562
10% SDS PAGE
GTX124214 diluted at 1:10000

GTX124214_40751_IHC_18121410_480.jpg

GTX124214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta actin(GTX124214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169