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beta Actin antibody

KO/KD
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214
Anti-beta Actin antibody used in Western Blot (WB). GTX124214

Cat. No. GTX124214

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

NIH-3T3 , Jurkat , Raji , K562 , 293T

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177721

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000.

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX124214 diluted at 1:10000

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214

GTX124214 IHC-P Image

beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta actin antibody (GTX124214) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124214

GTX124214 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using beta actin(GTX124214) antibody at 1:500 dilution.

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Sample (30 ug of whole cell lysate)
A: Jurakt
B: Raji
C: K562
10% SDS PAGE
GTX124214 diluted at 1:10000

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX124214

GTX124214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta actin(GTX124214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Actin antibody used in Western Blot (WB). GTX124214

GTX124214 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Act5C is the homolog of mammalian beta Actin. (Gene ID: 31521/ UniProt: P10987)

REFERENCE

Application Reference

De Tomi E et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Zanr? V et al. Int J Mol Sci 2022; 23 (3) Salviolone from Salvia miltiorrhiza Roots Impairs Cell Cycle Progression, Colony Formation, and Metalloproteinase-2 Activity in A375 Melanoma Cells: Involvement of P21(Cip1/Waf1) Expression and STAT3 Phosphorylation.
Application : WB
Reactivity : Human
Jacobs CL et al. Nat Methods 2018; 15 (7) : 523-526 StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins.
Peeva V et al. Nat Commun 2018; 9 (1) : 1727 Linear mitochondrial DNA is rapidly degraded by components of the replication machinery.
Application : WB
Reactivity : Human
Chung HK et al. Nat Chem Biol 2015; 11 (9) : 713-20 Tunable and reversible drug control of protein production via a self-excising degron.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169