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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX21801

Cat. No. GTX21801

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Goat, Sheep, Bovine, Hamster, Guinea pig, Pig, Monkey
Package
100 μg ($399)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:4000
ICC/IF 1:500 - 1:2000
ELISA 1:10000 - 1:40000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa whole cell lysate or mouse brain lysate.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: KQEYDESGPSC conjugated to KLH, corresponding to amino acids 359-368 of Human beta Actin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX21801

GTX21801 WB Image

Western Blot of Rabbit Anti-Beta Actin Antibody (GTX21801). Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 μg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4ºC. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none

REFERENCE

Application Reference

Matsuda N et al. J Pharmacol Exp Ther 2010; 332 (3) : 730-7 Up-regulation of histamine H4 receptors contributes to splenic apoptosis in septic mice: counteraction of the antiapoptotic action of nuclear factor-kappaB.
Application : WB
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399