GeneTex
United States (US)

beta Actin antibody

GTX30632_1443_WB_18121410_879.jpg
GTX30632_1441_WB_18121410_285.jpg
GTX30632_1442_WB_18121410_785.jpg
GTX30632_1444_WB_18121410_433.jpg
GTX30632_1440_WB_18121410_467.jpg
GTX30632_564_IHC-P_18121410_284.jpg

Cat No. GTX30632

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse, Rat, Sheep, Bovine, Dog, Chicken, Guinea pig, Pig, Horse, Rhesus Monkey, Turkey, Xenopus, Fish, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000 - 1:15000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Predict Reactivity

Chimpanzee, Gorilla, Orangutan, Panda(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A region corresponding to the N-terminus of human Beta Actin [UniProt# P60709]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm,cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30632_1443_WB_18121410_879.jpg

GTX30632 WB Image

WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates in NETN lysis buffer using GTX30632 beta Actin antibody.
Loading : 50μg
Dilution : 1 μg/ml

GTX30632_1441_WB_18121410_285.jpg

GTX30632 WB Image

WB analysis of HeLa cell lysate using GTX30632 beta Actin antibody.

GTX30632_1442_WB_18121410_785.jpg

GTX30632 WB Image

WB analysis of HepG2 cell lysate using GTX30632 beta Actin antibody.

GTX30632_1444_WB_18121410_433.jpg

GTX30632 WB Image

WB analysis of RCC4 cell lysate using GTX30632 beta Actin antibody.

GTX30632_1440_WB_18121410_467.jpg

GTX30632 WB Image

WB analysis of NIH-3T3 cell lysate using GTX30632 beta Actin antibody.
Lane 1 : 1:15,000
Lane 2 : 1:10,000
Lane 3 : 1:15,000
Loading : 20μg

GTX30632_564_IHC-P_18121410_284.jpg

GTX30632 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX30632 beta Actin antibody.
Dilution : 1 μg/ml

REFERENCE
Package List Price ($)
$ 369