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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX30632
Anti-beta Actin antibody used in Western Blot (WB). GTX30632
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX30632
Anti-beta Actin antibody used in Western Blot (WB). GTX30632
Anti-beta Actin antibody used in Western Blot (WB). GTX30632
Anti-beta Actin antibody used in Western Blot (WB). GTX30632

Cat. No. GTX30632

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Sheep, Bovine, Dog, Chicken, Guinea pig, Pig, Horse, Rhesus Monkey, Turkey, Xenopus, Fish, Primate
Package
100 μl ($649)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000 - 1:15000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Predict Reactivity

Chimpanzee, Gorilla, Orangutan, Panda(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A region corresponding to the N-terminus of human Beta Actin [UniProt# P60709]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_626093

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX30632

GTX30632 WB Image

WB analysis of RCC4 cell lysate using GTX30632 beta Actin antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX30632

GTX30632 WB Image

WB analysis of NIH-3T3 cell lysate using GTX30632 beta Actin antibody.
Lane 1 : 1:15,000
Lane 2 : 1:10,000
Lane 3 : 1:15,000
Loading : 20μg

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX30632

GTX30632 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX30632 beta Actin antibody.
Dilution : 1 μg/ml

Anti-beta Actin antibody used in Western Blot (WB). GTX30632

GTX30632 WB Image

WB analysis of HeLa cell lysate using GTX30632 beta Actin antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX30632

GTX30632 WB Image

WB analysis of HepG2 cell lysate using GTX30632 beta Actin antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX30632

GTX30632 WB Image

WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates in NETN lysis buffer using GTX30632 beta Actin antibody.
Loading : 50μg
Dilution : 1 μg/ml

REFERENCE

Application Reference

Bakirtzi K et al. J Immunol 2016; 196 (10) : 4311-21 Neurotensin Promotes the Development of Colitis and Intestinal Angiogenesis via Hif-1α-miR-210 Signaling.
Application : WB
Reactivity : Human
Chen KC et al. Cancer Genomics Proteomics 2016; 13 (3) : 219-230 dual roles of 17-β * in estrogen receptor-dependent growth inhibition in renal cell carcinoma.
Application : WB
Reactivity : Human
Taube JH et al. J Biol Chem 2010; 285 (21) : 16135-44 Foxa1 functions as a pioneer transcription factor at transposable elements to activate Afp during differentiation of embryonic stem cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 649