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beta Actin antibody

Anti-beta Actin antibody used in Western Blot (WB). GTX85117
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85117

Cat. No. GTX85117

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human beta-actin.The immunogen is located within amino acids 90 - 140 of Beta-actin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX85117

GTX85117 WB Image

WB analysis of HeLa cell lysate using GTX85117 beta Actin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85117

GTX85117 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85117 beta Actin antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399