beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
Green: beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Dot blot analysis of 100 ng beta Amyloid 1-42 oligomers or monomers, using beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at 1:10000, 1:20000, 1:40000, and 1:80000 dilution.

Indirect ELISA analysis was performed by coating plate with 100 μL of beta Amyloid (1-42) peptide aggregate at concentration of 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at concentrations ranging from 3.06 to 2230 ng/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:2000 and used to detect the primary antibody.

Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.