GeneTex
United States (US)

beta Amyloid (1-42) antibody – Conformation Specific

Comparison
GTX134510_43166_20180911_IHC-P_M_Tg-AD_18121410_759.jpg
GTX134510_43166_20180622_IHC-P_18121410_807.jpg
GTX134510_43166_20180316_IHC-P_1_18121410_683.jpg
GTX134510_43166_20180522_ELISA_18121410_507.jpg
GTX134510_43166_20180622_IHC-P_2_18121410_488.jpg
GTX134510_43166_20180403_DOT_LinCY_18121410_665.jpg

Cat No. GTX134510

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P, Dot, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
Dot Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Specificity/Sensitivity

This antibody specifically recognizes beta amyloid aggregates but not peptide monomers.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.45mg/ml

Antigen Species

Human

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AAA antibody , ABETA antibody , ABPP antibody , AD1 antibody , APP antibody , APPI antibody , Beta amyloid antibody , CTFgamma antibody , CVAP antibody , PN II antibody , PN2 antibody , amyloid beta precursor protein antibody

Cellular Localization

Membrane , Membrane , clathrin-coated pit

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
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Research Area

DATA IMAGES
GTX134510_43166_20180911_IHC-P_M_Tg-AD_18121410_759.jpg

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0
This image was provided courtesy of a customer review.

GTX134510_43166_20180622_IHC-P_18121410_807.jpg

GTX134510 IHC-P Image

Comparison

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510_43166_20180316_IHC-P_1_18121410_683.jpg

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510_43166_20180522_ELISA_18121410_507.jpg

GTX134510 ELISA Image

ELISA plate is coated with 100 µL of beta Amyloid (1-42) peptide aggregate at 10 µg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.

GTX134510_43166_20180622_IHC-P_2_18121410_488.jpg

GTX134510 IHC-P Image

Comparison

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510_43166_20180403_DOT_LinCY_18121410_665.jpg

GTX134510 Dot Image

Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.

REVIEW

beta Amyloid (1-42) antibody – Conformation Specific Cat No. GTX134510

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Sep-2018
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Hippocampus
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf