beta Amyloid (1-42) antibody – Conformation Specific

Name: beta Amyloid (1-42) antibody – Conformation Specific
Brand: GeneTex
SKU: GTX134510
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in ELISA (ELISA). GTX134510

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in Dot blot (Dot). GTX134510

Cat No. GTX134510

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	IHC-P, Dot, ELISA, Sandwich ELISA
Reactivity	Human, Mouse
Package	100 μl ($329), 25 μl ($169)

See all beta Amyloid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
IHC-P	1:100-1:1000
Dot	Assay dependent
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635160, Detection: GTX134510

Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Product Note

This antibody specifically recognizes beta amyloid aggregates but not peptide monomers.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human Beta amyloid (1-42)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887299

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

Cellular Localization

Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Database

Gene ID: 351 APP
UniProt: P05067 APP

Research Area

DATA IMAGES

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0
This image was provided courtesy of a customer review.

GTX134510 IHC-P Image

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510 ELISA Image

ELISA plate is coated with 100 μL of beta Amyloid (1-42) peptide aggregate at 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.

GTX134510 IHC-P Image

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX134510 ELISA Image

Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.

GTX134510 Dot Image

Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.

REFERENCE

Application Reference

Wang H et al. CNS Neurosci Ther 2021; (Epub) Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.

Application : IHC-P

Reactivity : Mouse

Dinkel F et al. Front Aging Neurosci 2020; 12 : 220 Decreased Deposition of Beta-Amyloid 1-38 and Increased Deposition of Beta-Amyloid 1-42 in Brain Tissue of Presenilin-1 E280A Familial Alzheimer's Disease Patients.

Application : IHC-P

Reactivity : Human

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