APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
| Assay dependent |
| 1:1000-1:10000 |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX635160, Detection: GTX134510
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Not tested in other applications.
Calculated MW
Product Note
This antibody specifically recognizes beta amyloid aggregates but not peptide monomers.
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen used to generate this antibody corresponds to human Beta amyloid (1-42)
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887299
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4
Cellular Localization
Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm
Background
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]
Database
Research Area
DATA IMAGES
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GTX134510 IHC-P Image
Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0
This image was provided courtesy of a customer review.
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GTX134510 IHC-P Image
Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500. *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134510 IHC-P Image
Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134510 ELISA Image
ELISA plate is coated with 100 μL of beta Amyloid (1-42) peptide aggregate at 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.
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GTX134510 IHC-P Image
Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500. *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134510 ELISA Image
Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.
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GTX134510 Dot Image
Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.
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REFERENCE
REVIEW
beta Amyloid (1-42) antibody – Conformation Specific
Cat. No. GTX134510
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Rating
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( Average 5 based on 1 users reviews)
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Application
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 10-Sep-2018
Application Tested :
IHC-P
Sample Species :
Ms
Sample :
Hippocampus
Primary Antibody :
1:500, 4°C, 16Hr
