APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:5000 |
| 1:50-1:200 |
| 1:50-1:200 |
| 1:50-1:200 |
| 1:10000-1:30000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4
Cellular Localization
Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm
Background
Beta Amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether Beta Amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of myloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of Beta Amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into Beta Amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Database
Research Area
DATA IMAGES
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GTX82974 WB Image
Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid (GTX82974) 1:1000 in 1% BSA-TBS-T o/n 4ºC. FEMTOMAX chemiluminescent substrate was used for detection of a 40-50 kD band consistent with a higher MW precursor form of beta amyloid. A secondary Ab only control (Shown right) showed no detectable signal.
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GTX82974 WB Image
Western Blot of Rabbit anti-Beta Amyloid Antibody. Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0 μg per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase Conjugated Goat-a-Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 40 kDa, 40 kDa for Beta Amyloid.
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GTX82974 IHC-Fr Image
Immunohistochemistical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added.
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GTX82974 ELISA Image
ELISA results of GTX82974 beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated and TMB Substrate.
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GTX82974 IHC-P Image
Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody (GTX82974) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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GTX82974 IHC-P Image
IHC-P analysis of human Alzheimer's disease brain tissue using GTX82974 beta Amyloid antibody.
Antigen retrieval : Heated mediated citrate buffer (pH 6.0)
Dilution : 1:100
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GTX82974 WB Image
WB analysis of various samples using GTX82974 beta Amyloid antibody.
Lane 1 : Protein ladder
Lane 2 : 293T whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : A-172 whole cell lysate
Lane 5 : Daudi whole cell lysate
Loading : 10 μg/mL
Dilution : 1:1000
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GTX82974 IHC-P Image
IHC-P analysis of human heart tissue using GTX82974 beta Amyloid antibody.
Dilution : 5 μg/mL
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GTX82974 WB Image
WB analysis of various samples using GTX82974 beta Amyloid antibody.
Lane 1 : Protein ladder
Lane 2 : 293T whole cell lysate
Lane 3 : Mouse brain tissus lysate
Lane 4 : A-172 whole cell lysate
Loading : 10 μg
Dilution : 1 μg/mL
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GTX82974 ELISA Image
ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX82974 beta Amyloid antibody.
Coating : 0.1 μg
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GTX82974 ICC/IF Image
ICC/IF analysis of MeOH-fixed HeLa cells using GTX82974 beta Amyloid antibody.
Green : Primary antibody
Blue : DAPI
APP is a cell surface protein that rapidly becomes internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles. Colocalizes with other proteins in a vesicular pattern in cytoplasm and perinuclear regions.
Dilution : 1 μg/mL
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GTX82974 WB Image
WB analysis of various samples using GTX82974 beta Amyloid antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : LNCaP whole cell lysate
Lane 7 : A-172 whole cell lysate
Lane 8 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 1:5000
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REFERENCE
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