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beta Arrestin 1 antibody [GT1126]

Recombinant antibody Orthogonal
Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318
Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318
Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318
Anti-beta Arrestin 1 antibody [GT1126] used in IHC (Paraffin sections) (IHC-P). GTX64318
Anti-beta Arrestin 1 antibody [GT1126] used in Flow cytometry (FACS). GTX64318
Anti-beta Arrestin 1 antibody [GT1126] used in IHC (Paraffin sections) (IHC-P). GTX64318

Cat. No. GTX64318

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1126

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all beta Arrestin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

TBS pH7.4, 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Arrestin Beta 1 , Arb1 , Arr1 , Arrb1

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Research Area

DATA IMAGES

Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318

GTX64318 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Arrestin 1 antibody [GT1126] (GTX64318) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 17965263

Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318

GTX64318 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Arrestin 1 antibody [GT1126] (GTX64318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Arrestin 1 antibody [GT1126] used in Western Blot (WB). GTX64318

GTX64318 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Arrestin 1 antibody [GT1126] (GTX64318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta Arrestin 1 antibody [GT1126] used in IHC (Paraffin sections) (IHC-P). GTX64318

GTX64318 IHC-P Image

IHC-P analysis of human lung tissue using GTX64318 beta Arrestin 1 antibody [GT1126].

Anti-beta Arrestin 1 antibody [GT1126] used in Flow cytometry (FACS). GTX64318

GTX64318 FACS Image

FACS analysis of HeLa cells using GTX64318 beta Arrestin 1 antibody [GT1126].
Blue : Primary antibody
Red : Isotype control

Anti-beta Arrestin 1 antibody [GT1126] used in IHC (Paraffin sections) (IHC-P). GTX64318

GTX64318 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX64318 beta Arrestin 1 antibody [GT1126].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399