APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1 μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
synthesized phosphopeptide derived from the region of rat beta-arrestin-1 that contains serine 412.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
arrestin, beta 1 , BARRES
Cellular Localization
Cytoplasm,Nucleus,Cell membrane,Cytoplasmic vesicle
Background
Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.
Database
Research Area
DATA IMAGES
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GTX24472 WB Image
WB analysis of tissue extracts (30 μg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2) using GTX24472 beta Arrestin 1 (phospho Ser412) antibody.
Dilution : 0.5 μg/ml
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GTX24472 WB Image
WB analysis of PC12 cell extracts were either not treated (Lane 1, 3) or treated (Lane 2, 4) with PP2A phosphatase using GTX24472 beta Arrestin 1 (phospho Ser412) antibody(Lane 3, 4) or b-arrestin-1 pan antibody(Lane 1, 2).
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REFERENCE
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REVIEW
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