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beta Catenin antibody [1F3]

Overexpression
Anti-beta Catenin antibody [1F3] used in Western Blot (WB). GTX84650
Anti-beta Catenin antibody [1F3] used in Western Blot (WB). GTX84650
Anti-beta Catenin antibody [1F3] used in IHC (Paraffin sections) (IHC-P). GTX84650

Cat. No. GTX84650

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F3

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all beta Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P 1:50
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 531-781 of human beta-catenin (NP_001895) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system. Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation.

Database

Research Area

DATA IMAGES

Anti-beta Catenin antibody [1F3] used in Western Blot (WB). GTX84650

GTX84650 WB Image

Overexpression

WB analysis of HEK293T cells transfected with beta Catenin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84650 beta Catenin antibody [1F3].
Loading : 5 ug per lane

Anti-beta Catenin antibody [1F3] used in Western Blot (WB). GTX84650

GTX84650 WB Image

WB analysis of various cell lines using GTX84650 beta Catenin antibody [1F3].
Loading : 35 ug per lane

Anti-beta Catenin antibody [1F3] used in IHC (Paraffin sections) (IHC-P). GTX84650

GTX84650 IHC-P Image

IHC-P analysis of human lung tissue using GTX84650 beta Catenin antibody [1F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519