beta Catenin antibody [4D5]

Cat. No. GTX83185

Host	Mouse
Clonality	Monoclonal
Clone Name	4D5
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX83185 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CTNNB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]

Database

Gene ID: 1499 CTNNB1
UniProt: P35222 CTNNB1

Research Area

DATA IMAGES

GTX83185 WB Image

WB analysis of beta Catenin -hIgGFc transfected HEK293 cell lysate using GTX83185 beta Catenin antibody [4D5].

GTX83185 IHC-P Image

IHC-P analysis of human Placenta tissue using GTX83185 beta Catenin antibody [4D5].

GTX83185 ICC/IF Image

ICC/IF analysis of A549 (left) and SK-BR-3 (right) cells using GTX83185 beta Catenin antibody [4D5].
Green : beta Catenin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX83185 FACS Image

FACS analysis of A549 cells using GTX83185 beta Catenin antibody [4D5].
Green : beta Catenin
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty