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beta Catenin antibody [6F9]

Anti-beta Catenin antibody [6F9] used in IHC (Paraffin sections) (IHC-P). GTX34441

Cat No. GTX34441

Host Mouse
Clonality Monoclonal
Clone Name 6F9
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Bovine, Dog, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

Recombinant chicken ?-catenin

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Catenin Beta 1,Ctnnb,Evr7,Mrd19,Armadillo,Ctnnb1

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
Anti-beta Catenin antibody [6F9] used in IHC (Paraffin sections) (IHC-P). GTX34441

GTX34441 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (6F9).

Package List Price ($)
$ 329