APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human beta Catenin.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catenin beta 1 , CTNNB , CTNNB1 , MRD19 , armadillo , beta Catenin , Catenin-β , β Catenin
Cellular Localization
Cytoplasm,Nucleus,Cytoskeleton,Cell junction,Adherens junction,Cell membrane,Microtubule organizing center,Centrosome,Spindle pole,Synapse
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/templates/default/js/lightbox/images/loading.gif "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
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GTX00948 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT1186] (GTX00948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00948 WB Image
WB analysis of HeLa whole cell lysate using GTX00948 beta Catenin antibody [GT1186].
Dilution : 1:1000
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GTX00948 WB Image
WB analysis of Mouse brain tissue lysate using GTX00948 beta Catenin antibody [GT1186].
Dilution : 1:1000
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GTX00948 IHC-P Image
IHC-P analysis of Human colon carcinoma tissue section using GTX00948 beta Catenin antibody [GT1186].
Dilution : 1:100
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GTX00948 WB Image
WB analysis of wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts using GTX00948 beta Catenin antibody [GT1186].
Dilution : 1:1000
Laoding : 25μg
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GTX00948 ICC/IF Image
ICC/IF analysis of PFA-fixed C6 cells using GTX00948 beta Catenin antibody [GT1186].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
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GTX00948 IHC-P Image
IHC-P analysis of Rat pancreas tissue section using GTX00948 beta Catenin antibody [GT1186].
Dilution : 1:100
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REFERENCE
There are currently no references for beta Catenin antibody [GT1186] (GTX00948). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for beta Catenin antibody [GT1186] (GTX00948). Be the first to share your experience with this product.
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_4000000136_20200313_WB_w_23053121_906.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_WB_1_w_23053121_214.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_WB_2_w_23053121_898.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_IHC-P_16_w_23053121_984.webp "Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_WB_3_w_23053121_371.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_ICC-IF_17_w_23053121_133.webp "Anti-beta Catenin antibody [GT1186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948](/upload/website/prouct_img/small/GTX00948/GTX00948_20200327_IHC-P_15_w_23053121_745.webp "Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_4000000136_20200313_WB_w_23053121_906.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_WB_1_w_23053121_214.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_WB_2_w_23053121_898.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_IHC-P_16_w_23053121_984.webp "Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_WB_3_w_23053121_371.webp "Anti-beta Catenin antibody [GT1186] used in Western Blot (WB). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_ICC-IF_17_w_23053121_133.webp "Anti-beta Catenin antibody [GT1186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00948")
![Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948](/upload/website/prouct_img/normal/GTX00948/GTX00948_20200327_IHC-P_15_w_23053121_745.webp "Anti-beta Catenin antibody [GT1186] used in IHC (Paraffin sections) (IHC-P). GTX00948")