beta Catenin antibody [GT3171]

Name: beta Catenin antibody [GT3171]
Brand: GeneTex
SKU: GTX632676
Price: 399.00 USD

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676

Anti-beta Catenin antibody [GT3171] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632676

Cat. No. GTX632676

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT3171
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all beta Catenin products

View product citations for antibody GTX632676 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , HeLa

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Database

Gene ID: 1499 CTNNB1
UniProt: P35222 CTNNB1

Research Area

DATA IMAGES

GTX632676 WB Image

Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX632676 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.

GTX632676 IHC-P Image

beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Red: beta Catenin stained by beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody (GTX632676) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX632676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.

GTX632676 IHC-P Image

beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody [GT3171] (GTX632676) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632676 ICC/IF Image

beta Catenin antibody detects beta Catenin protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:1000.

GTX632676 IHC-P Image

beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632676 IHC-P Image

beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632676 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX632676) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632676 WB Image

REFERENCE

Application Reference

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.

Application : WB

Reactivity : Human, Mouse

Submit a Reference

REVIEW

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Application Reference

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.

Application : WB

Reactivity : Human, Mouse

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

beta Catenin antibody [GT3171]

Cat. No. GTX632676

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX632676 WB Image

GTX632676 WB Image

GTX632676 IHC-P Image

GTX632676 WB Image

GTX632676 WB Image

GTX632676 IHC-P Image

GTX632676 ICC/IF Image

GTX632676 IHC-P Image

GTX632676 IHC-P Image

GTX632676 WB Image

GTX632676 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.