APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT116 , HeLa
Predict Reactivity
Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888327
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo
Cellular Localization
Cytoplasm , Nucleus , cytoskeleton
Background
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX632676 WB Image
Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX632676 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.
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GTX632676 IHC-P Image
beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Red: beta Catenin stained by beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632676 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody (GTX632676) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX632676 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.
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GTX632676 IHC-P Image
beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [GT3171] (GTX632676) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632676 ICC/IF Image
beta Catenin antibody detects beta Catenin protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:1000.
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GTX632676 IHC-P Image
beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632676 IHC-P Image
beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632676 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX632676) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632676 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX632676) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
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