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beta Catenin antibody [HL1753]

Anti-beta Catenin antibody [HL1753] used in Western Blot (WB). GTX637403
Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403
Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403
Anti-beta Catenin antibody [HL1753] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637403
Anti-beta Catenin antibody [HL1753] used in Western Blot (WB). GTX637403
Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403

Cat. No. GTX637403

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1753

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human beta Catenin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-beta Catenin antibody [HL1753] used in Western Blot (WB). GTX637403

GTX637403 WB Image

Wild-type (WT) and CTNNB1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403

GTX637403 IHC-P Image

beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403

GTX637403 IHC-P Image

beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [HL1753] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637403

GTX637403 ICC/IF Image

beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Catenin antibody [HL1753] used in Western Blot (WB). GTX637403

GTX637403 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Catenin antibody [HL1753] used in IHC (Paraffin sections) (IHC-P). GTX637403

GTX637403 IHC-P Image

beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for beta Catenin antibody [HL1753] (GTX637403). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for beta Catenin antibody [HL1753] (GTX637403). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations