APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Observed MW
92 kDa.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein of human beta Catenin
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo
Cellular Localization
Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
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GTX637403 WB Image
Wild-type (WT) and CTNNB1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637403 IHC-P Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637403 IHC-P Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat mammary gland.beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637403 IHC-P Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded dog mammary gland.beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637403 ICC/IF Image
beta Catenin antibody [HL1753] detects beta Catenin protein at cell membrane and cell junction by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin stained by beta Catenin antibody [HL1753] (GTX637403) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637403 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [HL1753] (GTX637403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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