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beta Catenin antibody [N1N2-2], N-term

KO/KD
GTX101435_42235_20151209_IHC-P_M_19011506_946.jpg
GTX101435_42235_20151209_IHC-P_M_2_19011506_175.jpg
GTX101435_42592_20161129_IHC-P_R_19011506_476.jpg
GTX101435_42536_20160830_IHC-P_M_19011506_903.jpg
GTX101435_39876_ChIP_19011506_649.jpg
GTX101435_39876_IHC_M_19011506_978.jpg
GTX101435_39876_WB_R_19011506_243.jpg
GTX101435_42669_20161228_IFA_19011506_422.jpg
GTX101435_42452_20160803_IHC-P_M_19011506_466.jpg
GTX101435_42452_20160713_IHC-P_H2_19011506_978.jpg
GTX101435_42235_20160205_IP_19011506_992.jpg
GTX101435_42235_20160715_FACS_19011506_591.jpg
GTX101435_39876_IHC_M_2_19011506_950.jpg
GTX101435_39876_20150206_IHC_M_3_19011506_736.jpg
GTX101435_42536_20160817_IFA_19011506_370.jpg
GTX101435_42564_20161129_IHC-P_M_19011506_618.jpg
GTX101435_42627_20161129_IHC-P_R_19011506_386.jpg
GTX101435_39876_WB_M_Brain_19011506_291.jpg
GTX101435_39876_IFA_19011506_155.jpg
GTX101435_42536_20161124_WB_19011506_374.jpg
GTX101435_42235_20160623_WB_shRNA_watermark_19011506_178.jpg
GTX101435_42452_20160713_IHC-P_H_19011506_996.jpg

Cat No. GTX101435

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:50-1:100
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HeLa , A549 , H1299 , HCT116 , PC-12 , Mouse brain , *human fibroblast , *LX2

Predict Reactivity

Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTNNB antibody, DKFZp686D02253 antibody, FLJ25606 antibody, FLJ37923 antibody, CTNNB1 antibody, catenin beta-1 antibody, catenin (cadherin-associated protein), beta 1, 88kDa antibody

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
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Database

Research Area

DATA IMAGES
GTX101435_42235_20151209_IHC-P_M_19011506_946.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42235_20151209_IHC-P_M_2_19011506_175.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42592_20161129_IHC-P_R_19011506_476.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42536_20160830_IHC-P_M_19011506_903.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_39876_ChIP_19011506_649.jpg

GTX101435 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

GTX101435_39876_IHC_M_19011506_978.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435_39876_WB_R_19011506_243.jpg

GTX101435 WB Image

beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract |
7.5% SDS-PAGE
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435_42669_20161228_IFA_19011506_422.jpg

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101435_42452_20160803_IHC-P_M_19011506_466.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42452_20160713_IHC-P_H2_19011506_978.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42235_20160205_IP_19011506_992.jpg

GTX101435 IP Image

Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody (GTX101435).
Western blot analysis was performed using beta Catenin antibody (GTX101435).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101435_42235_20160715_FACS_19011506_591.jpg

GTX101435 FACS Image

beta Catenin antibody [N1N2-2], N-term (GTX101435) detects CTNNB1 protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX101435_39876_IHC_M_2_19011506_950.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435_39876_20150206_IHC_M_3_19011506_736.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101435_42536_20160817_IFA_19011506_370.jpg

GTX101435 ICC/IF Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101435_42564_20161129_IHC-P_M_19011506_618.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_42627_20161129_IHC-P_R_19011506_386.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101435_39876_WB_M_Brain_19011506_291.jpg

GTX101435 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101435 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435_39876_IFA_19011506_155.jpg

GTX101435 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101435_42536_20161124_WB_19011506_374.jpg

GTX101435 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000.

GTX101435_42235_20160623_WB_shRNA_watermark_19011506_178.jpg

GTX101435 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101435_42452_20160713_IHC-P_H_19011506_996.jpg

GTX101435 IHC-P Image

beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

beta Catenin antibody [N1N2-2], N-term Cat No. GTX101435

Rating ( Average 4 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 3 users reviews)
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Lane Description : Lane 1: 293T cells, Lane 2-3: knockdown of β Catenin in 293T cells
Date : Anonymous submitted on 28-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : LX2 cells
Amount used : 10μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : Lane 1: LX2 cells.Lane 2: LX2 cells (TGF-beta treatment).
Date : Anonymous submitted on 31-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : fibroblast cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169