beta Catenin antibody [rCTNNB1/2173]

Cat. No. GTX17731

Host	Mouse
Clonality	Monoclonal
Clone Name	rCTNNB1/2173
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, Protein Array
Reactivity	Human, Mouse, Rat
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX17731 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human '-catenin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTNNB , CTNNB1 , MRD19 , armadillo , catenin beta 1 , beta Catenin , Catenin-β , β Catenin

Cellular Localization

Cytoplasm,Nucleus,Cytoskeleton,Cell junction,Adherens junction,Cell membrane,Microtubule organizing center,Centrosome,Spindle pole,Synapse

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Database

Gene ID: 1499 CTNNB1
UniProt: P35222 CTNNB1

Research Area

DATA IMAGES

GTX17731 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX17731 beta Catenin antibody [rCTNNB1/2173].
Green : Primary antibody
Red : nucleus

GTX17731 WB Image

WB analysis of HeLa cell lysate using GTX17731 beta Catenin antibody [rCTNNB1/2173].

GTX17731 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX17731 beta Catenin antibody [rCTNNB1/2173].

GTX17731 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Beta-Catenin Mouse Recombinant Monoclonal Antibody (rCTNNB1/2173). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty