GeneTex
  • Country / Location Selection

United States (US)

beta Catenin antibody

Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254
Anti-beta Catenin antibody used in Western Blot (WB). GTX101254
Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254
Anti-beta Catenin antibody used in Western Blot (WB). GTX101254
Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254
Anti-beta Catenin antibody used in Western Blot (WB). GTX101254

Cat No. GTX101254

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A549 , mouse brain , HeLa

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

catenin beta 1,CTNNB,EVR7,MRD19,armadillo

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254

GTX101254 IHC-P Image

beta Catenin antibody detects CTNNB1 protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
beta Catenin antibody (GTX101254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Catenin antibody used in Western Blot (WB). GTX101254

GTX101254 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX101254 diluted at 1:1000

Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254

GTX101254 IHC-P Image

beta Catenin antibody detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody (GTX101254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Catenin antibody used in Western Blot (WB). GTX101254

GTX101254 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101254) diluted at 1:1000.

Anti-beta Catenin antibody used in IHC (Paraffin sections) (IHC-P). GTX101254

GTX101254 IHC-P Image

beta Catenin antibody detects beta Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody (GTX101254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Catenin antibody used in Western Blot (WB). GTX101254

GTX101254 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101254 diluted at 1:1000

REFERENCE
REVIEW
There are currently no reviews for beta Catenin antibody (GTX101254). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169