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beta Catenin (phospho Ser33/Ser37/Thr41) antibody

Orthogonal
Anti-beta Catenin (phospho Ser33/Ser37/Thr41) antibody used in Western Blot (WB). GTX132605

Cat. No. GTX132605

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all beta Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HeLa (100 nM Calyculin A treatment for 0.5 hr)

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser33/Ser37/Thr41 of human beta Catenin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886691

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm , Nucleus , cytoskeleton

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta Catenin (phospho Ser33/Ser37/Thr41) antibody used in Western Blot (WB). GTX132605

GTX132605 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser33/Ser37/Thr41) antibody (GTX132605) diluted at 1:500.

REFERENCE

Application Reference

Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Liu J et al. Mol Med Rep 2019; 20 (1) : 33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169