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beta Catenin (phospho Ser33/Ser37) antibody [BC-22]

Orthogonal
Anti-beta Catenin (phospho Ser33/Ser37) antibody [BC-22] used in Western Blot (WB). GTX11350

Cat. No. GTX11350

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BC-22

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat, Chicken
Package
50 μl ($399)
See all beta Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 5-10 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HEK-293 cells treated with proteasome inhibitor MG132

Product Note

The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_368987

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR.

Database

Research Area

DATA IMAGES

Anti-beta Catenin (phospho Ser33/Ser37) antibody [BC-22] used in Western Blot (WB). GTX11350

GTX11350 WB Image

Orthogonal

The data was published in the journal PLoS Biol in 2018.PMID: 29337987

REFERENCE

Application Reference

Chou YT et al. PLoS Biol 2018; 16 (1) : e2003714 Identification of a noncanonical function for ribose-5-phosphate isomerase A promotes colorectal cancer formation by stabilizing and activating β-catenin via a novel C-terminal domain.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399