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beta Catenin (phospho Ser37) antibody

Anti-beta Catenin (phospho Ser37) antibody used in Western Blot (WB). GTX50256
Anti-beta Catenin (phospho Ser37) antibody used in IHC (Paraffin sections) (IHC-P). GTX50256

Cat. No. GTX50256

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all beta Catenin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 37 (I-H-S(p)-G-A) derived from human beta Catenin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11173758

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-beta Catenin (phospho Ser37) antibody used in Western Blot (WB). GTX50256

GTX50256 WB Image

WB analysis of extracts from HT-29 cells using GTX50256 beta Catenin (phospho Ser37) antibody with or without blocking peptide.

Anti-beta Catenin (phospho Ser37) antibody used in IHC (Paraffin sections) (IHC-P). GTX50256

GTX50256 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50256 beta Catenin (phospho Ser37) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Xie B et al. Mol Med Rep 2019; 20 (2) : 1808-1818 Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β?catenin pathway.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399