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beta-Gal antibody [N2C3]

Anti-beta-Gal antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104360
Anti-beta-Gal antibody [N2C3] used in Western Blot (WB). GTX104360

Cat No. GTX104360

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta-Gal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036345

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galactosidase beta 1 , EBP , ELNR1 , MPS4B

Cellular Localization

Isoform 1: Lysosome , Isoform 2: Cytoplasm , perinuclear region

Background

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta-Gal antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104360

GTX104360 IHC-P Image

beta-Gal antibody [N2C3] detects beta-Gal protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
beta-Gal antibody [N2C3] (GTX104360) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta-Gal antibody [N2C3] used in Western Blot (WB). GTX104360

GTX104360 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Gal antibody [N2C3] (GTX104360) diluted at 1:1000.

REFERENCE

REVIEW

beta-Gal antibody [N2C3] Cat No. GTX104360

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27281159
Date : Anonymous submitted on 05-Jul-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : PANC-1 cells
Blocking : PBS with 2% FBS and 0.4% Triton X-100, 25°C, 0.17Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : PBS with 2% FBS and 0.4% Triton X-100
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169