GeneTex
United States (US)

beta-Gal antibody [N2C3]

GTX104360_WB_19011506_919.jpg
GTX104360_39925_IHC_19011506_815.jpg

Cat No. GTX104360

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Cat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta-Gal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EBP antibody, ELNR1 antibody, MPS4B antibody, GLB1 antibody, acid beta-galactosidase antibody, beta-galactosidase antibody, lactase antibody, "elastin receptor 1, 67kDa antibody", "galactosidase, beta 1 antibody"

Cellular Localization

Isoform 1: Lysosome , Isoform 2: Cytoplasm , perinuclear region

Background

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX104360_WB_19011506_919.jpg

GTX104360 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX104360 diluted at 1:1000

GTX104360_39925_IHC_19011506_815.jpg

GTX104360 IHC-P Image

beta-Gal antibody [N2C3] detects beta-Gal protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
beta-Gal antibody [N2C3] (GTX104360) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

beta-Gal antibody [N2C3] Cat No. GTX104360

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : PANC-1 cells
Blocking : PBS with 2% FBS and 0.4% Triton X-100, 25°C, 0.17Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : PBS with 2% FBS and 0.4% Triton X-100
Primary Antibody : 1:100, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169