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beta-Gal antibody [OTI5H2]

Overexpression
Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880
Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880
Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880
Anti-beta-Gal antibody [OTI5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04880

Cat. No. GTX04880

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OTI5H2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Dog, Monkey
Package
100 μl ($539)
See all beta-Gal products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS,1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GLB1(NP_001073279) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EBP,ELNR1,GLB1,MPS4B,galactosidase beta 1

Cellular Localization

Lysosome,Cytoplasm,Perinuclear region

Background

This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880

GTX04880 WB Image

Overexpression

WB analysis of HEK293T cells with/without human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2].
Loading : 5 μg

Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880

GTX04880 WB Image

ICC/IF analysis of COS7 cells with human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2].

Anti-beta-Gal antibody [OTI5H2] used in Western Blot (WB). GTX04880

GTX04880 WB Image

WB analysis of various samples using GTX04880 beta-Gal antibody [OTI5H2].
Loading : 10 μg
Dilution : 1:200

Anti-beta-Gal antibody [OTI5H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04880

GTX04880 ICC/IF Image

FACS analysis of HEK293T cells with/without human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2].
Red : HEK293T cells with human GLB1 transfection
Blue line : HEK293T cells without human GLB1 transfection

REFERENCE

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REVIEW

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Package List Price ($)
$ 539