beta Galactosidase antibody (FITC)

Cat. No. GTX26641
Cat. No.  GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

WB analysis of E.coli beta Galactosidase protein using GTX26641 beta Galactosidase antibody (FITC).
Lane 1 : 80 ng
Lane 2 : 20 ng
Dilution : 1:10000

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

WB analysis of E.coli beta Galactosidase protein using GTX26641 beta Galactosidase antibody (FITC).
Loading : 1 μg
Dilution : 1:1000

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

WB analysis of various samples using GTX26641 beta Galactosidase antibody (FITC).
Lane 1 : E.coli beta Galactosidase (60 ng)
Lane 2 : E.coli beta Galactosidase (30 ng)
Lane 3 : E.coli beta Galactosidase (15 ng)
Lane 4 : E. coli lysate
Dilution : 1:10000

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

Western blotting using GeneTex anti-beta-Galactosidase antibody (GTX26641). Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4º C with Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with anti-b-Galactosidase diluted to 1:10,000. Reaction occurred overnight at 4ºC. Dylight649™ conjugated Gt-a-anti-Rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center).in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

Western blotting using GeneTex Fluorescein conjugated anti-b-Galactosidase antibody (GTX26641) shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase (GTX26641) diluted to 1:10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M.Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
GTX26641 WB Image

Western blot using GeneTex anti-beta Galactosidase antibody (GTX26641) shows detection of a band at ~117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1μg of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4º C followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

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Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
Anti-beta Galactosidase antibody (FITC) used in Western Blot (WB). GTX26641
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF
  • Reactivity
    E. coli
See all beta Galactosidase products