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beta Galactosidase antibody

Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX77365
Anti-beta Galactosidase antibody used in Immunohistochemistry (IHC). GTX77365

Cat. No. GTX77365

Reference 14

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, IHC

Reactivity

 Mouse, Drosophila, E. coli
Package
100 μg ($399)
See all beta Galactosidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2 - 0.05 ug/ml
ICC/IF 10 - 2 ug/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
IHC 1.0 - 5.0 ug/ml
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Chickens were immunized with purified beta-galactosidase from Escherichia coli. After multiple immunizations in Freunds adjuvant, eggs were collected and delipidated. Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-ga

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_379834

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ECK0341 , JW0335

Database

Research Area

DATA IMAGES

Anti-beta Galactosidase antibody used in ELISA (ELISA). GTX77365

GTX77365 ELISA Image

ELISA analysis of immunogen using GTX77365 beta Galactosidase antibody.

Anti-beta Galactosidase antibody used in Immunohistochemistry (IHC). GTX77365

GTX77365 IHC Image

IHC analysis of immunogen using GTX77365 beta Galactosidase antibody.

REFERENCE

Application Reference

Fan A et al. Bone Res 2023; 11 (1) : 3 Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression.
Emmanuel Martin et al. Dev Cell 2021; 56 (5) : 687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Reactivity : Drosophila
Jonathan M Pojer et al. PLoS Genet 2021; 17 (6) : e1009146 Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Sun J et al. Nat Commun 2021; 12 (1) : 4611 SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts.
Application : IHC-P
Olinski LE et al. eNeuro 2020; 7 (5) Endogenous Opsin 3 (OPN3) Protein Expression in the Adult Brain Using a Novel OPN3-mCherry Knock-In Mouse Model.
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REVIEW

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Application Reference
Fan A et al. Bone Res 2023; 11 (1):3 Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression.
Emmanuel Martin et al. Dev Cell 2021; 56 (5):687-701.e7 Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.
Reactivity : Drosophila
Jonathan M Pojer et al. PLoS Genet 2021; 17 (6):e1009146 Crumbs and the apical spectrin cytoskeleton regulate R8 cell fate in the Drosophila eye.
Sun J et al. Nat Commun 2021; 12 (1):4611 SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts.
Application : IHC-P
Olinski LE et al. eNeuro 2020; 7 (5) Endogenous Opsin 3 (OPN3) Protein Expression in the Adult Brain Using a Novel OPN3-mCherry Knock-In Mouse Model.
Wei H et al. Cell Rep 2020; 33 (2):108252 Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Application : IHC-Fr
Reactivity : Mouse
Ji G et al. Bone Joint J 2019; 101-B (7_Supple_C):108-114 Vascular endothelial growth factor pathway promotes osseointegration and CD31hiEMCNhi endothelium expansion in a mouse tibial implant model: an animal study.
Application : IHC-Fr
Ghiglione C et al. Development 2018; (Epub) the drosophila * pathway controls profilin expression and dynamic actin-rich protrusions during collective cell migration.
Xu R et al. Nat Med 2018; 24 (6):823-833 Targeting skeletal endothelium to ameliorate bone loss.
Application : IHC-Fr
Agrawal N et al. Cell Metab 2016; 23 (4):675-84 the drosophila tnf eiger is an adipokine that acts on *-producing cells to mediate nutrient response.
Application : ICC/IF
Monier B et al. Nature 2015; 518 (7538):245-8 Apico-basal forces exerted by apoptotic cells drive epithelium folding.
Application : WB
Matusek T et al. Nature 2014; 516 (7529):99-103 The ESCRT machinery regulates the secretion and long-range activity of Hedgehog.
Jouandin P et al. Development 2014; 141 (15):3013-21 Starvation induces FoxO-dependent mitotic-to-endocycle switch pausing during Drosophila oogenesis.
Application : IHC
Gettings M et al. PLoS Biol 2010; 8 (6):e1000390 JNK signalling controls remodelling of the segment boundary through cell reprogramming during Drosophila morphogenesis.
Application : IHC
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399