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beta Galactosidase antibody

Anti-beta Galactosidase antibody used in Western Blot (WB). GTX85284

Cat. No. GTX85284

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 E. coli
Package
100 μg ($409)
See all beta Galactosidase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal.The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.

Research Area

DATA IMAGES

Anti-beta Galactosidase antibody used in Western Blot (WB). GTX85284

GTX85284 WB Image

WB analysis of (A) 5 and (B) 25 ng b-gal using GTX85284 beta Galactosidase antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409