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beta Galactosidase antibody

Anti-beta Galactosidase antibody used in Western Blot (WB). GTX85284

Cat No. GTX85284

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ELISA
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal.The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.

Research Area

DATA IMAGES

Anti-beta Galactosidase antibody used in Western Blot (WB). GTX85284

GTX85284 WB Image

WB analysis of (A) 5 and (B) 25 ng b-gal using GTX85284 beta Galactosidase antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409