beta-Hydroxy-GABA antibody

Cat. No. GTX134714
Cat. No.  GTX134714
Anti-beta-Hydroxy-GABA antibody used in ELISA (ELISA). GTX134714
GTX134714 ELISA Image

ELISA plate is coated with 100 μL of beta-hydroxy-GABA-carrier protein conjugate at 10 μg/mL. The coated ligand is detected with beta-hydroxy-GABA antibody (GTX134714) at concentrations ranging from 550 ng/mL down to 2.0 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) is diluted at 1:2,000 and used to detect the primary antibody.

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Anti-beta-Hydroxy-GABA antibody used in Dot blot (Dot). GTX134714
GTX134714 Dot Image

Dot blot analysis of 100 ng KLH-conjugated beta-hydroxy-GABA, using beta-hydroxy-GABA antibody (GTX134714) at 1:4000, 1:8000, and 1:16000.

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Anti-beta-Hydroxy-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134714
GTX134714 ICC/IF Image

beta-Hydroxy-GABA antibody detects beta-Hydroxy-GABA protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta-Hydroxy-GABA stained by beta-Hydroxy-GABA antibody (GTX134714) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-beta-Hydroxy-GABA antibody used in ELISA (ELISA). GTX134714
Anti-beta-Hydroxy-GABA antibody used in Dot blot (Dot). GTX134714
Anti-beta-Hydroxy-GABA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134714
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    ICC/IF Dot ELISA
  • Reactivity
    Rat
Summary
Antibody specificity was performed with a competitive ELISA test with the following compounds:
CompoundsCross-reactivity ratio
β-Hydroxy-GABA-G-CP1
Glycine-G-CP1/>10,000
β-Alanine-G-CP1/>10,000
Glutamine-G-CP1/>10,000
Glutamate-G-CP1/>10,000
Agmatine-G-CP1/>10,000

G: Glutaraldehyde, CP: Carrier Protein (Gelatin).
See all beta-Hydroxy-GABA products