beta-Hydroxy-GABA antibody

Cat. No. GTX134714

GTX134714 ELISA Image

ELISA plate is coated with 100 μL of beta-hydroxy-GABA-carrier protein conjugate at 10 μg/mL. The coated ligand is detected with beta-hydroxy-GABA antibody (GTX134714) at concentrations ranging from 550 ng/mL down to 2.0 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) is diluted at 1:2,000 and used to detect the primary antibody.

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GTX134714 Dot Image

Dot blot analysis of 100 ng KLH-conjugated beta-hydroxy-GABA, using beta-hydroxy-GABA antibody (GTX134714) at 1:4000, 1:8000, and 1:16000.

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GTX134714 ICC/IF Image

beta-Hydroxy-GABA antibody detects beta-Hydroxy-GABA protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta-Hydroxy-GABA stained by beta-Hydroxy-GABA antibody (GTX134714) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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This beta-Hydroxy-GABA antibody (GTX134714) is a rabbit polyclonal antibody validated for detection of beta-Hydroxy-GABA by ICC/IF, Dot, and ELISA. Reactivity is species-independent.

Host

Rabbit
Clonality

Polyclonal
Isotype

IgG
Applications

ICC/IF Dot ELISA
Reactivity

Species independent

Summary

Antibody specificity was performed with a competitive ELISA test with the following compounds:

Compounds	Cross-reactivity ratio
β-Hydroxy-GABA-G-CP	1
Glycine-G-CP	1/>10,000
β-Alanine-G-CP	1/>10,000
Glutamine-G-CP	1/>10,000
Glutamate-G-CP	1/>10,000
Agmatine-G-CP	1/>10,000

G: Glutaraldehyde, CP: Carrier Protein (Gelatin).