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beta Lactamase antibody [8A5.A10]

Cat. No. GTX12251

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A5.A10

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 E. coli
Package
500 μg ($499)
See all beta Lactamase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 10μg/ml
ELISA 10-20μg/ml (optimized for immobilized beta-lactamase at 10ug/ml)
Not tested in other applications.

Product Note

This antibody recognizes TEM-1-type beta-lactamases.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

5’-His-tagged E. coli 205 TEM-1 R+ beta-lactamase Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

REFERENCE

Application Reference

Takuya Miyake et al. Front Microbiol 2020; 11 : 607381 Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP.
Application : WB
Reactivity : E. coli
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SDS
PBS.pdf
Package List Price ($)
$ 499