Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP antibody [N1C1] (GTX102667) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP antibody [N1C1] (GTX102667) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta-TrCP antibody [N1C1] detects beta-TrCP protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
beta-TrCP antibody [N1C1] (GTX102667) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Oncotarget in 2016.PMID: 27034167