beta-TrCP antibody [N1C1]

Name: beta-TrCP antibody [N1C1]
Brand: GeneTex
SKU: GTX102667
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-beta-TrCP antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102667

Anti-beta-TrCP antibody [N1C1] used in Western Blot (WB). GTX102667

Cat. No. GTX102667

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX102667 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Mouse, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta-TrCP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949769

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-transducin repeat containing E3 ubiquitin protein ligase , BETA-TRCP , FBW1A , FBXW1 , FBXW1A , FWD1 , bTrCP , bTrCP1 , betaTrCP

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]

Database

Gene ID: 8945 BTRC
UniProt: Q9Y297 BTRC

Research Area

DATA IMAGES

GTX102667 IHC-P Image

beta-TrCP antibody [N1C1] detects beta-TrCP protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
beta-TrCP antibody [N1C1] (GTX102667) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102667 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP antibody [N1C1] (GTX102667) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102667 WB Image

The data was published in the journal Oncotarget in 2016.PMID: 27034167

REFERENCE

Application Reference

Kogami M et al. Oncol Lett 2023; 26 (1) : 313

Mariko Kogami et al. Oncol. Lett. 2023; 26 : 313

Application : WB

Reactivity : Human

Qin ZY et al. Cancer Res 2019; BRD4 promotes gastric cancer progression and metastasis through acetylation-dependent stabilization of Snail.

Application : IHC-P

Reactivity : Human

Yi-Chang Wang et al. Nature Communication 2018; 9 : 3996 USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.

Application : IHC-P

Reactivity : Human

Lin TP et al. Oncotarget 2016; (Epub) REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling.

Application : WB

Reactivity : Human

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