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beta-TrCP antibody

Anti-beta-TrCP antibody used in IHC (Paraffin sections) (IHC-P). GTX55536
Anti-beta-TrCP antibody used in Western Blot (WB). GTX55536
Anti-beta-TrCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55536

Cat No. GTX55536

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-340 of human BTRC (NP_378663.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-transducin repeat containing E3 ubiquitin protein ligase , BETA-TRCP , FBW1A , FBXW1 , FBXW1A , FWD1 , bTrCP , bTrCP1 , betaTrCP

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-beta-TrCP antibody used in IHC (Paraffin sections) (IHC-P). GTX55536

GTX55536 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55536 beta-TrCP antibody.
Dilution : 1:100

Anti-beta-TrCP antibody used in Western Blot (WB). GTX55536

GTX55536 WB Image

WB analysis of U-251MG cell lysate using GTX55536 beta-TrCP antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-beta-TrCP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55536

GTX55536 ICC/IF Image

ICC/IF analysis of C6 cells using GTX55536 beta-TrCP antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

There are currently no references for beta-TrCP antibody (GTX55536). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for beta-TrCP antibody (GTX55536). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339