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beta Tubulin 2 antibody [N1C1]

Anti-beta Tubulin 2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101863
Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863
Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863
Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863
Anti-beta Tubulin 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101863

Cat No. GTX101863

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all TUBB2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3 , mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241418

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 2A class IIa , CDCBM5 , TUBB , TUBB2

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101863

GTX101863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using beta Tubulin 2(GTX101863) antibody at 1:200 dilution.

Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863

GTX101863 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101863 diluted at 1:5000

Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863

GTX101863 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101863 diluted at 1:3000

Anti-beta Tubulin 2 antibody [N1C1] used in Western Blot (WB). GTX101863

GTX101863 WB Image

Sample (30 ug of whole cell lysate)

A: 293T

10% SDS PAGE

GTX101863 diluted at 1:1000

Anti-beta Tubulin 2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101863

GTX101863 IHC-P Image

beta Tubulin 2 antibody [N1C1] detects beta Tubulin 2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
beta Tubulin 2 antibody [N1C1] (GTX101863) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169