beta Tubulin 2 antibody [N1C1]

Cat No. GTX101863

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($379), 25 μl ($169)

See all TUBB2A products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3 , mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241418

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 2A class IIa , CDCBM5 , TUBB , TUBB2

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Gene ID: 7280 TUBB2A
UniProt: Q13885 TUBB2A

Research Area

DATA IMAGES

GTX101863 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using beta Tubulin 2(GTX101863) antibody at 1:200 dilution.

GTX101863 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101863 diluted at 1:5000

GTX101863 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101863 diluted at 1:3000

GTX101863 WB Image

Sample (30 ug of whole cell lysate)

A: 293T

10% SDS PAGE

GTX101863 diluted at 1:1000

GTX101863 IHC-P Image

beta Tubulin 2 antibody [N1C1] detects beta Tubulin 2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
beta Tubulin 2 antibody [N1C1] (GTX101863) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 379

25 μl

$ 169

Qty

beta Tubulin 2 antibody [N1C1]

Cat No. GTX101863

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX101863 ICC/IF Image

GTX101863 WB Image

GTX101863 WB Image

GTX101863 WB Image

GTX101863 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.