Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using beta Tubulin 2(GTX101863) antibody at 1:200 dilution.

beta Tubulin 2 antibody [N1C1] detects beta Tubulin 2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
beta Tubulin 2 antibody [N1C1] (GTX101863) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)

A: 293T

10% SDS PAGE

GTX101863 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101863 diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101863 diluted at 1:3000