APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , C8D30 , NIH3T3 , BCL-1 , Raw 264.7 , C2Cl2 , Drosophila lysate , rat brain
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.55mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
TUBB antibody, TUBB2 antibody, dJ40E16.7 antibody, TUBB2A antibody, "tubulin, beta polypeptide 2 antibody", tubulin beta-2A chain antibody, class IIa beta-tubulin antibody, "tubulin, beta 2A antibody"
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Database
DATA IMAGES
|
GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000
|
|
GTX100117 ICC/IF Image
TUBB2A antibody detects TUBB2A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: TUBB2A protein stained by TUBB2A antibody (GTX100117) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
GTX100117 WB Image
Sample (30 ug of whole cell lysate)
A: Drosophila lysate
10% SDS PAGE
GTX100117 diluted at 1:1000
|
|
GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000
|
|
GTX100117 IHC-P Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Tubulin 2 antibody (GTX100117) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000
|
|
GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extrac
C. 30 μg BCL-1 whole cell lysate/extrac
D. 30 μg Raw 264.7 whole cell lysate/extract
E. 30 μg C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000
|
REFERENCE
REVIEW
beta Tubulin 2 antibody
Cat No. GTX100117
|
Rating
|
|
( Average 4 based on 1 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 4 based on 1 users reviews)
|
Date :
Anonymous submitted on 25-Aug-2016
Application Tested :
WB
Sample Species :
Rat
Sample :
Rat brain tissue
Amount used :
25μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:100, 4°C, overnightHr
