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beta Tubulin 2 antibody

Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117
Anti-beta Tubulin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100117
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117
Anti-beta Tubulin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100117
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

Cat No. GTX100117

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($329),
25 μl ($169)
See all TUBB2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , C8D30 , NIH3T3 , BCL-1 , Raw 264.7 , C2Cl2 , Drosophila lysate , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 2A class IIa , CDCBM5 , TUBB , TUBB2

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

GTX100117 WB Image

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Anti-beta Tubulin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100117

GTX100117 ICC/IF Image

TUBB2A antibody detects TUBB2A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: TUBB2A protein stained by TUBB2A antibody (GTX100117) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

GTX100117 WB Image

Sample (30 ug of whole cell lysate)
A: Drosophila lysate
10% SDS PAGE
GTX100117 diluted at 1:1000

Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

GTX100117 WB Image

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Anti-beta Tubulin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100117

GTX100117 IHC-P Image

beta Tubulin 2 antibody detects beta Tubulin 2 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Tubulin 2 antibody (GTX100117) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

GTX100117 WB Image

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000

Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117

GTX100117 WB Image

beta Tubulin 2 antibody detects beta Tubulin 2 protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extrac
C. 30 μg BCL-1 whole cell lysate/extrac
D. 30 μg Raw 264.7 whole cell lysate/extract
E. 30 μg C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000

REFERENCE

Application Reference

Nichole M. Owen et al. 0 2015; PARP inhibitor : abrogation of chromosomal radial-induced mitotic cell death and induction of multipolar division via loss of centrosome integrity.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

beta Tubulin 2 antibody Cat No. GTX100117

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-beta Tubulin 2 antibody used in Western Blot (WB). GTX100117
Date : Anonymous submitted on 25-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain tissue
Amount used : 25μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 329
$ 169