APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , C8D30 , NIH-3T3 , BCL-1 , Raw 264.7 , C2Cl2 , rat brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10731273
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin beta 2A class IIa , CDCBM5 , TUBB , TUBB2
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Database
Research Area
DATA IMAGES
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GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 μg drosophila lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:2000
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GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:10000
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GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:5000
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GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:5000
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GTX100117 WB Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (GTX100117) dilution: 1:5000
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GTX100117 IHC-P Image
beta Tubulin 2 antibody detects beta Tubulin 2 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
beta Tubulin 2 antibody (GTX100117) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100117 ICC/IF Image
TUBB2A antibody detects TUBB2A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: TUBB2A protein stained by TUBB2A antibody (GTX100117) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
REVIEW
beta Tubulin 2 antibody
Cat. No. GTX100117
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 25-Aug-2016
Application Tested :
WB
Sample Species :
Rat
Sample :
Rat brain tissue
Amount used :
25μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:100, 4°C, 16Hr
