GeneTex
United States (US)

beta Tubulin 2C antibody [N2C2], Internal

GTX115403_40618_IHC_18121410_789.jpg
GTX115403_40618_WB_M_18121410_890.jpg
GTX115403_40618_WB_18121410_347.jpg

Cat No. GTX115403

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta 4B Class Ivb,Beta2,Lcaeod,Tubb2,Tubb2C,Tubb4B

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Research Area

DATA IMAGES
GTX115403_40618_IHC_18121410_789.jpg

GTX115403 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using beta Tubulin 2C(GTX115403) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115403_40618_WB_M_18121410_890.jpg

GTX115403 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115403 diluted at 1:5000

GTX115403_40618_WB_18121410_347.jpg

GTX115403 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115403 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169