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beta Tubulin 3/ Tuj1 antibody [5H2]

KO/KD Overexpression
Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460
Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460
Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460

Cat. No. GTX83460

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H2

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460

GTX83460 WB Image

KO/KD

WB analysis of wild type and beta Tubulin 3/ Tuj1 knock out HeLa cell lysate(10μg per lane) using GTX83460 beta Tubulin 3/ Tuj1 antibody [5H2].
Dilution : 1:500

Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460

GTX83460 WB Image

Overexpression

WB analysis of HEK293T cells transfected with beta Tubulin 3/ Tuj1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83460 beta Tubulin 3/ Tuj1 antibody [5H2].
Loading : 5 ug per lane

Anti-beta Tubulin 3/ Tuj1 antibody [5H2] used in Western Blot (WB). GTX83460

GTX83460 WB Image

WB analysis of various cell lines using GTX83460 beta Tubulin 3/ Tuj1 antibody [5H2].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519