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beta Tubulin 3/ Tuj1 antibody [GT11710]

KO/KD
GTX631836_41855_20160830_IHC-Fr_18121411_161.jpg
GTX631836_41855_20161004_IFA_18121411_461.jpg
GTX631836_41855_20160808_2_IHC-Fr_18121411_390.jpg
GTX631836_41855_20160808_1_IHC-Fr_18121411_202.jpg
GTX631836_41855_20150625_IHC_M_18121411_929.jpg
GTX631836_41855_20161208_WB_M_R_18121411_282.jpg
GTX631836_41855_42459_IHC-P_R_18121411_884.jpg
GTX631836_41855_20150625_IP_18121411_883.jpg
GTX631836_41855_20150618_WB_18121411_687.jpg
GTX631836_41855_20170309_WB_KO_watermark_18121411_844.jpg

Cat No. GTX631836

Host Mouse
Clonality Monoclonal
Clone Name GT11710
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Specificity/Sensitivity

beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta III Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CDCBM Antibody , CFEOM3A Antibody , TUBB4 Antibody , tubulin, beta 3 class III Antibody , beta-4 Antibody , TUBB3 Antibody, TUJ1 Antibody

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX631836_41855_20160830_IHC-Fr_18121411_161.jpg

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836_41855_20161004_IFA_18121411_461.jpg

GTX631836 ICC/IF Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836_41855_20160808_2_IHC-Fr_18121411_390.jpg

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836_41855_20160808_1_IHC-Fr_18121411_202.jpg

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836_41855_20150625_IHC_M_18121411_929.jpg

GTX631836 IHC-Fr Image

beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: DAPI

GTX631836_41855_20161208_WB_M_R_18121411_282.jpg

GTX631836 WB Image

Various tissue extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX631836_41855_42459_IHC-P_R_18121411_884.jpg

GTX631836 IHC-P Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX631836_41855_20150625_IP_18121411_883.jpg

GTX631836 IP Image

Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836).
Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX631836_41855_20150618_WB_18121411_687.jpg

GTX631836 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX631836_41855_20170309_WB_KO_watermark_18121411_844.jpg

GTX631836 WB Image

KO/KD

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

REFERENCE
REVIEW

beta Tubulin 3/ Tuj1 antibody [GT11710] Cat No. GTX631836

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 04-Jul-2016
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Retina tissue
Blocking : 20% normal donkey serum and 0.3% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4%Paraformaldehyde+0.01%Glutaraldehyde in PBS
Primary Antibody : 1:250, 4°C, 24Hr
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169