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beta Tubulin 3/ Tuj1 antibody [GT1338]

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831
Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831
Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831
Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831
Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831

Cat No. GTX631831

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1338

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , U87-MG , SK-N-SH , IMR32 , mouse brain , rat brain

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888259

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631831

GTX631831 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in IHC (Paraffin sections) (IHC-P). GTX631831

GTX631831 IHC-P Image

beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831

GTX631831 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831

GTX631831 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631831) diluted by 1:1000.

Anti-beta Tubulin 3/ Tuj1 antibody [GT1338] used in Western Blot (WB). GTX631831

GTX631831 WB Image

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169