beta Tubulin 3/ Tuj1 antibody [GT886]

Name: beta Tubulin 3/ Tuj1 antibody [GT886]
Brand: GeneTex
SKU: GTX631830
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-beta Tubulin 3/ Tuj1 antibody [GT886] used in IHC (Frozen sections) (IHC-Fr). GTX631830

Anti-beta Tubulin 3/ Tuj1 antibody [GT886] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631830

Anti-beta Tubulin 3/ Tuj1 antibody [GT886] used in Western Blot (WB). GTX631830

Anti-beta Tubulin 3/ Tuj1 antibody [GT886] used in IHC (Paraffin sections) (IHC-P). GTX631830

Anti-beta Tubulin 3/ Tuj1 antibody [GT886] used in Immunoprecipitation (IP). GTX631830

Cat. No. GTX631830

Host	Mouse
Clonality	Monoclonal
Clone Name	GT886
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX631830 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , mouse brain

Product Note

beta III Tubulin antibody [GT886] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2888258

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Gene ID: 10381 TUBB3
UniProt: Q13509 TUBB3

Research Area

DATA IMAGES

GTX631830 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631830 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

The staining pattern is based on the publication:PMID: 30390091

GTX631830 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631830) diluted at a dilution of 1:1000.

GTX631830 IHC-Fr Image

beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.

GTX631830 WB Image

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX631830 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.

GTX631830 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.

GTX631830 IHC-P Image

beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250.

GTX631830 IP Image

Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830).
Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

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REVIEW