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beta Tubulin 3/ Tuj1 antibody [HL1708]

Recombinant antibody Orthogonal
Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Western Blot (WB). GTX637307
Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637307
Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637307

Cat. No. GTX637307

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1708

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all beta Tubulin 3/ Tuj1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human beta Tubulin 3/ Tuj1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Western Blot (WB). GTX637307

GTX637307 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1708] (GTX637307) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637307

GTX637307 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody [HL1708] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.
Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1708] (GTX637307) diluted at 1:500.
Red: NF-H, a axon marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta Tubulin 3/ Tuj1 antibody [HL1708] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637307

GTX637307 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody [HL1708] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1708] (GTX637307) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations