APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide of human beta Tubulin 3/ Tuj1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
Cellular Localization
Cytoplasm, cytoskeleton
Background
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/templates/default/js/lightbox/images/loading.gif "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
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GTX637308 WB Image
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637308 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637308 ICC/IF Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637308 ICC/IF Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in ice-cold MeOH for 10 min.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637308 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637308 IHC-P Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637308 IHC-P Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308). Be the first to share your experience with this product.
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221125_WB_KO_watermark_22112723_537.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221111_WB_M_R_22111518_185.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221209_ICC_IF_R_22122018_965.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221230_ICC_IF_22122901_860.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230106_WB_TPM_watermark_23010922_324.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230217_IHC-P_R_23021401_499.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230217_IHC-P_M_23021401_223.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221125_WB_KO_watermark_22112723_537.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221111_WB_M_R_22111518_185.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221209_ICC_IF_R_22122018_965.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221230_ICC_IF_22122901_860.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230106_WB_TPM_watermark_23010922_324.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230217_IHC-P_R_23021401_499.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230217_IHC-P_M_23021401_223.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")