APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:400 |
Assay dependent |
Assay dependent |
Note :
WB
using MDCK cells fixed in methanol
|
Not tested in other applications.
Calculated MW
Positive Control
rat brain
Product Note
The antibody specifically recognizes an epitope located on isotype III of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. The antibody can stimulate microtubule assembly when reconstituted with tubulin and either tau or MAP2.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype III coupled to BSA
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
Cellular Localization
Cytoplasm, cytoskeleton
Background
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy-terminal. For beta-tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta- tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.
Database
Research Area
DATA IMAGES
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GTX11314 WB Image
WB analysis of (1)U87 (2) MDBK (3) rat2 cell lysate using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400 .
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GTX11314 WB Image
WB analysis of rat brain cytosol extract using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10].
Lane A : Antibody dilution 1:400
Lane B : Negative control (only secondary antibody)
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GTX11314 ICC/IF Image
ICC/IF analysis of SH-SY-5Y cells using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400(pink) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
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REFERENCE
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REVIEW
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