Flow Cytometry: Use at an assay dependant concentration
Recommended dilution:1-2 μg/ml, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
Positive material: Neuro2a mouse neuroblastoma cell line
Optimal dilutions/concentrations should be determined by the end user.
Class III beta-tubulin specific for neurones.
Phosphate buffered saline pH 7.4 containing 15mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Synthetic peptide: ESESQGPK conjugated to malemide activated KLH, corresponding to amino acids 441-448 of Human Tubulin.
Purified by precipitation methods
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Tubulin Beta 3 Class Iii,Cdcbm,Cdcbm1,Cfeom3,Cfeom3A,Feom3,Tubb4,Beta-4,Tubb3
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]