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beta Tubulin 3/ Tuj1 antibody [TU-20]

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunohistochemistry (IHC). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Flow cytometry (FACS). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Flow cytometry (FACS). GTX27751
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunohistochemistry (IHC). GTX27751

Cat No. GTX27751

Host Mouse
Clonality Monoclonal
Clone Name TU-20
Isotype IgG1
Application WB, ICC/IF, FACS, IHC
Reactivity Human, Mouse, Rat, Bovine, Hamster, Pig, Baboon, Carp
APPLICATION

Application Note

Flow Cytometry: Use at an assay dependant concentration
Western Blotting
Recommended dilution:1-2 μg/ml, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
Immunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line
Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

Class III beta-tubulin specific for neurones.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide: ESESQGPK conjugated to malemide activated KLH, corresponding to amino acids 441-448 of Human Tubulin.

Purification

Purified by precipitation methods

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Beta 3 Class Iii,Cdcbm,Cdcbm1,Cfeom3,Cfeom3A,Feom3,Tubb4,Beta-4,Tubb3

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunohistochemistry (IHC). GTX27751

GTX27751 IHC Image

GTX27751 at a dilution of 1/1000, staining neuron specific beta III tubulin TU-20 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Image shows labeling pf n

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27751

GTX27751 ICC/IF Image

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
2B - Merged image of co-staining with anti-beta-tubulin (GTX27792).
Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Flow cytometry (FACS). GTX27751

GTX27751 FACS Image

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (GTX27751; green; 3 μg/ml). Nuclei were stained with DAPI (blue)

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Flow cytometry (FACS). GTX27751

GTX27751 FACS Image

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (GTX27751; red).

Anti-beta Tubulin 3/ Tuj1 antibody [TU-20] used in Immunohistochemistry (IHC). GTX27751

GTX27751 IHC Image

GTX27751 at a dilution of 1/1000, staining neuron specific beta III tubulin TU-20 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images shows cytoskeletal

REFERENCE
Package List Price ($)
$ 319