beta Tubulin 3/ Tuj1 antibody

Name: beta Tubulin 3/ Tuj1 antibody
Brand: GeneTex
SKU: GTX129913
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-beta Tubulin 3/ Tuj1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129913

Anti-beta Tubulin 3/ Tuj1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129913

Anti-beta Tubulin 3/ Tuj1 antibody used in Western Blot (WB). GTX129913

Cat. No. GTX129913

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all beta Tubulin 3/ Tuj1 products

View product citations for antibody GTX129913 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , SK-N-SH , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

Database

Gene ID: 10381 TUBB3
UniProt: Q13509 TUBB3

Research Area

DATA IMAGES

GTX129913 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129913 ICC/IF Image

beta Tubulin 3/ Tuj1 antibody detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody (GTX129913) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX129913 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at a dilution of 1:10000.

GTX129913 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody (GTX129913) diluted at 1:10000.

REFERENCE

Application Reference

Kazuya Furusawa et al. Researh square 2023; A cerebral organoid autonomically maintains the shape of the organic muscle cell actuators

Kogo Y et al. Biophys Physicobiol 2020; 17 : 132-139 Rapid differentiation of human dental pulp stem cells to neuron-like cells by high K+ stimulation.

Application : ICC/IF

Reactivity : Human

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